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KMID : 0613820020120040610
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Journal of Life Science
2002 Volume.12 No. 4 p.610 ~ p.621
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Investigation of Conserved Gene in Microbial Genomes using in silico Analysis
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Kang Ho-Young
Shin Chang-Jin
Kang Byung-Chul
Park Hyung-Chul
Shin Dong-Hoon
Choi Jung-Hyun
Cho Hwan-Gyu
Cha Jae-Hoo
Lee Dong-Geun
Lee Jae-Hwa
Park Hee-Kyung
Kim Cheol-Min
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Abstract
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Conserved genes are importantly used to understand the major function in survival and replication of living organism. This study was focused on identification of conserved genes in microbial species and measuring the degree of conservation. For this purpose, in silico analysis was performed to search conserved genes based on the conservation level within microbial species. The ortholog list of COGs (Clusters of Orthologous Groups of proteins) in NCBI was used and whole genomes of 43 microbial species were included in that list. The distance value, derived from CLUSTALW multiple alignment program, was used as a descriptor of the conservation level of orthologs. It was revealed that 43 microbial genomes hold 72 conserved orthologs in common. The majority(72.2%) of the conserved genes was related to "translation, ribosomal structure and biogenesis" functional category. A GTPase-translation elogation factor(COG0050) was the best conserved gene from the distance value analysis. The 72 conserved genes, found in this research, would be useful not only to study minimal function genes but also new drug target among pathogens and to make a model of the virtual cell.
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KEYWORD
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conserved gene, microbial genome, ortholog, COG, minimal function gene, in silico analysis
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